Preparation of ultra-competent E. coli cells for transformation

Liquid culture of Arabidopsis and chitin treatment

Agrobacterium mediated rice transformation

Biolistic-transformation of rice
 

Arabidopsis transformation by vacuum infiltration

RT-PCR

Northern Blotting

Gus staining

Fluorimetric GUS-assay

Midi RNA preparation using Trizol reagent

Electroporation of E.Coli or Agrobacterium

Plant DNA isolation

Molecular weight of chitin oligomers and Nod factors

Bacterial mating with Bradyrhizobium (prepared by Joyce Yuen, Apr. 9, 1997)

Liquid culture of Arabidopsis and chitin treatment

PROTOCOL FOR EXTRACTION OF NOD FACTORS (LCOs) FROM INDUCED BRADYRHIZOBIUM JAPONICUM CULTURES USING RESINS

Recipe for B&D (Broughton and Dilworth 1971) nutrient solution for growing legumes in Leonard jars and pots

ISOLATION OF BRADYOXETIN

ISOLATION OF SOYBEAN SEEDLING NolA INDUCER

Microscopy and Immunolocalization of GS50 and GS52